Plasma Membrane Domains Participate in pH Banding of Chara Internodal Cells

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منابع مشابه

Plasma Membrane Domains Participate in pH Banding of Chara Internodal Cells

We investigated the identity and distribution of cortical domains, stained by the endocytic marker FM 1-43, in branchlet internodal cells of the characean green algae Chara corallina and Chara braunii. Co-labeling with NBD C(6)-sphingomyelin, a plasma membrane dye, which is not internalized, confirmed their location in the plasma membrane, and co-labelling with the fluorescent pH indicator Lyso...

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Involvement of membrane potential in alkaline band formation by internodal cells of Chara corallina.

Internodal cells of Chara corallina form alkaline bands on their surface upon illumination via photosynthesis. In the present study, the effect of KCl on alkaline band formation was analyzed. When the extracellular KCl concentration was increased, alkaline band formation was extensively inhibited. Electrophysiological analysis unequivocally showed the need for inner negative membrane potential ...

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Autonomous Local Area Control over Membrane Transport in Chara Internodal Cells.

Internodal cells of Chara were separated with a Plexiglas divider into two segments and the vibrating probe was used to investigate the extracellular current profiles that formed along these two surfaces. Treating one segment of the Chara cell with K(+) concentrations greater than 2 millimolar caused a dramatic reduction in the extracellular current pattern in this compartment. Concentrations o...

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Convoluted Plasma Membrane Domains in the Green Alga Chara are Depleted of Microtubules and Actin Filaments

Charasomes are convoluted plasma membrane domains in the green alga Chara australis. They harbor H(+)-ATPases involved in acidification of the medium, which facilitates carbon uptake required for photosynthesis. In this study we investigated the distribution of cortical microtubules and cortical actin filaments in relation to the distribution of charasomes. We found that microtubules and actin ...

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Induction of a new alkaline band at a target position in internodal cells of Chara corallina.

Characean cells develop alternating alkaline and acid bands on their surface upon illumination. However, the mechanism of band formation is not fully understood. In the present study, we succeeded in inducing a new alkaline band at an original acid band in internodal cells of Chara corallina. Chloroplasts in an acid band were locally removed by wounding the cell in the absence of the cell turgo...

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ژورنال

عنوان ژورنال: Plant and Cell Physiology

سال: 2011

ISSN: 1471-9053,0032-0781

DOI: 10.1093/pcp/pcr074